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Detection of Enterococcal asa1 and vanA Genes in Clinical Samples from Adult Immunocompromised Patients

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1Nesreen M. Kamel*, 2Mai E. Ahmed, 3Mohamed N. Salem, 4Ahmed F. Azmy,
5Ehab M. Fahmy
1Department of Clinical Pathology, 2Department of Clinical Pathology and 3Department of Internal Medicine Faculty of Medicine – Beni-Suef University, Beni-suef, Egypt, 4Department of Microbiology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt and 5Department of Medical Microbiology and Immunology, Aswan Faculty of Medicine, Aswan, Egypt

ABSTRACT

Background: Vancomycin resistant Enterococci (VRE) is important opportunistic nosocomial pathogens due to rapid spread, and limited treatment options. Objectives: The aims of this study were to assess incidence of enterococcal infections and VRE in adult immunocompromised patients and detection of asa1 and vanA genes among these isolates. Methodology: The study included 80 adult immunocompromised patients admitted to Beni-Suef University Hospital. Antimicrobial susceptibility was done by disc diffusion method then MIC was done for vancomycin resistant strains and Enterococcus was considered VRE if the MIC was ≥ 16μg/ml. PCR was done for detection of vanA and asa1 genes. Results: Enterococci were detected in 36 samples, 22.2% of isolates were vancomycin resistant. vanA and asa1 were detected in 33.3% and 63.8% of isolates respectively. VanA gene was detected in 4 (50%) of VRE whereas vanA gene was detected in 4 VSE isolates. Comclusion: Further studies are needed for detection of other virulence and resistance genes and their role in bacterial pathogenicity.

About EJMM

The Egyptian Journal of Medical Microbiology EJMM  ISSN Print 1110-2179 - ISSN Online: 1110-2179, The official publication of Egyptian Society of Medical Microbiology

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